For years, scientists speculated that the heart’s helical arrangements and complex musculature pumps blood more efficiently throughout the body. Though thanks to the difficulty in reproducing such complex and intricate details in artificial hearts, researching this phenomenon proved difficult.

However, in a new study published in Science, a team of researchers from multiple institutions have come together to utilize a new, more advanced method to fabricate artificial tissues and organs. This team included Qihan Liu, an assistant professor in the University of Pittsburgh Swanson School of Engineering.

“It is a huge manufacturing challenge to make material that mimics biological tissue,” Liu said. “Biological tissues consist of various micro/nanofibers of complex spatially varying alignment. The arrangement of these fibers is how nature program the rich mechanical behavior of biological tissue. However, currently there is no manufacturing tool that can capture this complex arrangement. While common 3D printing methods can easily realize complex fiber arrangements, 3D printing cannot print nanofibers at a throughput relevant to tissue engineering. While established nanofiber spinning techniques such as electrospinning can produce nanofibers at mass-production-level high throughput, complex fiber arrangement has yet to be realized.”

The researchers thus proposed the process of focused rotary jet spinning. FRJS is an additive manufacturing method which uses centrifugal spinning to rapidly form fibers. The fibers are subsequently focused, aligned, and deposited onto targeted locations with a controlled airstream. By using airflow, this method allows thousands of micro/nanofibers to be simultaneously manipulated, which ensures high throughput. With the specially designed airflow, FRJS can manufacture 3D fiber constructs that are much more complex than current methods can create.

The researchers compare their methods to two other ways of producing artificial organs and tissue: 3D printing and fiber spinning. Although 3D printing continues to contribute major advancements to the development of artificial organs and tissue, the researchers counter that 3D printing takes too long to achieve the detail and complexity FRJS can offer.

Another currently used method is fiber spinning, which allows for a faster production but not as much detail or complexity as FRJS. Thus, the researchers present FRJS as a method to make more complex creations in a timelier manner.

To display the possibilities of FRJS in this context, the researchers used the process to create samples, including a full-scale fiber human heart model to show the capability of manufacture complex 3D structure, a three layer dual-chamber ventricle model to show the capability to capture complex fiber alignment in biological tissues and single-layer ventricles with the size of a rat, cat, human and the 12,000-pound minke whale to show the scalability of the manufacturing process. The researchers also demonstrate how models like these created by FRJS can be used to understand the function of the helical cell arrangements within the heart.

These samples, however, are just one display of what can be done with FRJS.

“This paper is just one demonstration of what can be done with this method, really,” Liu said. “The sky is the limit.”

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